Brian Chait, Rockefeller University - Butler Seminar Speaker 3-12-2021
"Addressing Challenging Problems in Proteomic Research”
Abstract: Although mass spectrometry-based proteomics is facilitating many new avenues of biological research, this remarkable set of tools is still limited in its applications. I will discuss some of these limitations and our efforts to address them. In particular, I will address methods for accurately modeling large macromolecular assemblies that may be flexible and undergo conformational changes; capturing specific interactions that are weak and/or transient; elucidating dynamic macromolecular processes, identifying large repertoires of tightly binding antibodies against targeted antigens, as well as addressing limitations in the signal-to-noise ratio and dynamic range of current mass spectrometric technologies.