Eusociality is characterized by incredible organization and reproductive success, yet it requires the vast majority of members of the society to sacrifice their own reproductive capacity for the greater good. Because of this, eusociality has interested evolutionary biologist seeking to identify its mechanisms. In fact, Charles Darwin called Eusocial insect societies a "challenge" to his theory of natural selection. Bumble bees represent a middle ground of eusociality because they are less organized than highly eusocial species like honeybees, but still have a queen and division of labor. In addition, their response to juvenile hormone (JH) is characteristic of both solitary and eusocial species. For this reason, JH is an interesting avenue of research as a mechanism for the formation of Eusociality on a biological level. My summer research focused on treating bumble bees with JH and measuring how it changed the expression of certain genes in their brains.