Toxoplasma gondii is a single-cell parasite which causes toxoplasmosis, a disease causing flu-like symptoms in healthy individuals but severe eye or brain damage in congenitally infected children, and death in immunodeficient individuals. The CDC estimates that over 40 million Americans may be infected with this parasite transmitted via undercooked meats and cat feces. Toxoplasmosis is a serious global health problem that becomes increasingly difficult to treat as the parasite develops drug resistance. My research focuses on an organelle fission protein in T. gondii which is thought to direct a process crucial to the parasite’s ability to divide and infect further host cells. I aim to investigate this protein which holds great importance in the development of new therapeutics against T. gondii using structural and biophysical approaches. The protein of interest, expressed and purified from a model system, is studied via methods including analytical ultracentrifugation, lipid blot assays, and fluorescence microscopy. These provide insight to the protein behavior regarding both its interactions with itself to form aggregates at different concentrations, and its interactions with the lipid bilayer of organelle membranes. The nature of this agent as an enzyme (a protein that quickens cellular reactions) is also explored through experiments that estimate the enzymatic efficiency for a protein. Analyzing this protein has great potential to inform the development of effective drug inhibitors of a protein-membrane interaction essential to the propagation of the parasite — potentially the cure for a disease posing health risks to millions of immunocompromised individuals, mothers, and congenitally infected newborns.