Territory exploration in animals is conducive to gain knowledge about investigated environments and evaluate social information driven by recognition of chemosensory cues. Previously, it was known that male mice display territorial behavior with urine markings and use chemosensory cues to guide exploration of novel environments. However, it is not well researched if females display similar territory expression to males. We recorded female and male mice locomotion in a territory arena assay that featured three removable walls to divide the arena into resident, neutral, and intruder occupied areas. From our results we found that males explore resident, neutral, and intruder regions at unequal proportions and females were not found to have differences in occupation between the three regions. We found a significant difference between the proportion of time spent in the resident and intruder occupied zones between males and females. We found these differences in exploration patterns may be explained by differences in urinary marking between sex. To further explore the possible neural correlates involved during territory exploration, we then used wireless electrophysiology technology to record single unit activity in the lateral septum as this region has been implicated in a diverse array of functions including space encoding, social information processing, affect, and motivation. We recorded neural activity in the LS in both female and male mice when alternating between home and novel cages. Our results found selective firing patterns for each cage in both sexes.